Functional genomics of organelles
Maya Schuldiner Lab
Our Laboratory's Research
Functional genomics of organelles
One of the hallmarks of eukaryotic cells is the presence of membrane-enclosed organelles that create optimized environments best suited for promoting the various chemical reactions required to sustain life. Although more than 15 years have passed since the publication of the Saccharomyces cerevisiae genome sequence, over 30% of the proteins that reside in its organelles have never been studied and more than half of them do not have a known biochemical function. Most of these proteins are conserved all the way to humans and some have been implicated in diseases. One of the great challenges of the post-genomic era is, therefore, to use novel methodologies to fill in these gaps in our knowledge, to uncover the functions of these unstudied proteins, and to delineate pathways and networks that enable the function and communication of these organelles. Our lab is dedicated to uncovering novel functions for yeast organellar proteins. We do this by employing a wide variety of high throughput screening techniques complemented by dedicated cell biological, genetic and biochemical follow ups.
Will robots take over the world?
Understanding how the ER is organized into subdomains
Emma is interested in the endoplasmic reticulum (ER) and how this extensive organelle, built from one continuous membrane, is compartmentalized into specialized regions required for its diverse functions. To do this, she is using different technologies to map distinct regions and the proteins that reside in them.
Uncovering the targeting pathway for mitochondrial ribosomal proteins lacking a canonical MTS
Yury studies how a subset of mitochondrial ribosomal proteins (MRPs) are transported into mitochondria. While most proteins functioning in the mitochondrial matrix have a mitochondrial targeting sequence (MTS) that is cleaved off after import, many of the MRPs do not have a canonical MTS. Using systematic genetic screens and microscopy Yury is trying to figure out what is special about the import of these MRPs, which signals do they use and what is the protein machinery required to target and translocate them.
Development of a fast diagnostic test for peroxisomal diseases
Ofir is screening a library of fluorescent compounds to identify a synthetic dye which specifically labels peroxisomes. The dye will be tested for its universality and ease of use. Identifying a peroxisomal dye will simplify staining and imaging procedures of peroxisomes for research and significantly expand the diagnostic toolkit for peroxisomal diseases.
Development of Multi-CLEM in mammalian
Sarah is developing a high-throughput screening approach (multi-CLEM) for electron microscopy in mammalian cells. The combination of fluorescence microscopy for barcoding of tens or hundreds of differently manipulated cells and electron microscopy will allow quantitative imaging at the ultrastructure level.
Uncovering new targeting factors of proteins to the ER
Sivan is interested in how proteins with unique targeting signals are targeted to the Endoplasmic Reticulum (ER) in yeast. Sivan hopes to discover new factors involved in the process. To do this, she uses high throughput screens of mutant libraries combined with image analysis.
Discovering New Peroxisomal Proteins Via a Novel Fluorescent Genomic Library
Lior is interested in using new fluorescently tagged genomic libraries for the identification of new peroxisomal proteins. Lior wishes to uncover the targeting pathways that they take to reach peroxisomes and elucidate their biological features and function.
Uncovering new membrane shaping proteins in mitochondria
Noga is interested in the unique structure of mitochondria, forming a continuous branched network in the cell. Using high throughput screens and microscopy, Noga aims to discover new membrane shaping proteins that enable the formation of this intricate structure.
ERC CoG 646604