During the last decade, biology has gone through a technology-driven revolution with the emergence of high-throughput methods. Yeast has been an excellent model organism for these efforts, allowing the collection of large amounts of systematic data. However, there remains a huge gap between the amount of data generated and its ability to put forward high quality predictions that can be followed up to obtain true mechanistic understanding of cellular functions. Our lab aims to bridge the gap by using high throughput methods in combination with detailed follow up to provide mechanistic understanding of fundamental unresolved questions in cell biology.