The SWAp-Tag method allows for easy replacing of a tag in a parental strain collection with an expression cassette of choice to rapidly create new yeast libraries in order to address biological questions in Saccharomyces cerevisiae.
The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of modified strains (libraries). The Swap-Tag method was designed to overcome inherent limitations in library construction. The method (SWAT) relies on one parental library that can be modified easily and efficiently to give rise to an endless variety of libraries of choice. The library is composed of ~5,800 strains carrying a SWAT-GFP module at the amino termini of endomembrane proteins, and we are working on preparing a full genome collection. We also possess two other libraries made by the SWAT approach- mCherry and seamless GFP. These libraries, as well as query strains and constructs are freely available upon request.